Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint
نویسندگان
چکیده
منابع مشابه
Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint
Seventeen mint accessions representing the three species grown for commercial oil production in the United States were characterized using randomly amplified polymorphic DNA (RAPD) analysis. The RAPD profiles readily identified the different Mentha species; calculation of genetic distance, based on the number of shared bands, indicated that M. spicata L. is more closely related to M. x gracilis...
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Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
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Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...
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The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose...
متن کاملMethod to improve reliability of random-amplified polymorphic DNA markers.
Random-amplified polymorphic DNA (RAPD) markers (8) are a powerful tool for genome analysis (7). Nevertheless, the difficulties to achieve a high pattern repeatability represent a major drawback for the routine implementation of RAPD markers. Amplification products obtained depend on various factors, such as the concentration of the reaction mixture constituents, the source of Taq DNA polymeras...
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ژورنال
عنوان ژورنال: HortScience
سال: 2001
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.36.4.761